A simple and sensitive UV-spectrophotometric method has been developed for simultaneous determination of Salbutamol sulphate, Ambroxol HCl and Dextromethorphan HBr in a combined pharmaceutical dosage form. In the proposed method, the absorbances were measured at 224 nm 244 nm and 277 nm corresponding to the absorbance maxima of Salbutamol sulphate, Ambroxol HCl and Dextromethorphan HBr in methanol respectively. Linearity range was observed in the concentration range of 10-50 μg/ml. Concentration of each drug was obtained by using the absorptivities calculated for all three drugs at wavelengths, 224 nm, 244 nm and 277 nm and solving the simultaneous equation. Developed method was applied to pharmaceutical formulation. The method was validated statistically and recovery study was performed to confirm the accuracy of the method. The method was found to be rapid, simple, accurate and precise.
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